EasyPure® RNA Kit
Referência ER101-01
Tamanho : 50rxns
Marca : TransGen Biotech
Contactar o distribuidor local :
Telefone : +1 850 650 7790
ManualEasyPure® RNA Kit provides a simple and fast column based method to isolate total RNA from animal cells, animal tissues, bacteria and yeast. Cells and tissues are enzymatically lysed. DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted from the column. RNA is free of protein contamination, and is suitable for RT-PCR, qRT-PCR and Northern blot.
Storage
DNase I and DNase I Reaction Buffer at -20°C for one year; others at room temperature (15°C-25°C) in a dry place for one year.
Shipping
DNase I and DNase I Reaction Buffer on dry ice (-70 °C); Others at room temperature.
| Component | ER101-01 (50 rxns) |
| Binding buffer 4 (BB4) | 40 ml |
| Clean buffer 4 (CB4) | 60 ml |
| Wash buffer 4 (WB4) | 12 ml |
| Proteinase K (20 mg/ml) | 1 ml |
| DNase I (3 units/μl) | 1500 units |
| DNase I Reaction Buffer | 4×1 ml |
| RNase-free Water | 10 ml |
| RNase-free Tube (1.5 ml) | 50 each |
| RNA Spin Columns with Colle ction Tub e s | 50 each |
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