Images
Result Data
Note

Result Data

Result Data

Detection of Caspase 9 Activities in Jurkat cells. Jurkat cells were seeded at 200,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with 1uM staurosporine for 5 hours while the untreated cells were used as control. The caspase 9 assay loading solution (100 uL/well) was added and incubated at room temperature for 1hour. The fluorescence intensity was measured at Ex/Em = 370/450 nm with a FlexStation microplate reader (Molecular Devices).

  • Specifications

    Product Description

    CASP9 Apoptosis Kit (Blue Fluorescence) is a fluorescent assay used to monitor cell apoptosis by measuring CASP9 activity.

    Suitable Sample

    Adherent cells, Non-adherent cells

    Detection Method

    Fluorometric

    Excitation (Max)

    370 nm

    Emission (Max)

    450 nm

    Regulation Status

    For research use only (RUO)

    Storage Instruction

    Store the kit at -20°C and avoid from light.

    Note

    Result Data

  • Applications

    Functional Study