Component | AS401-01 | AS401-02 | AS401-03 |
2×TransStart® GoldPfu PCR SuperMix (-dye) | 1 ml | 5×1 ml | 15×1 ml |
Nuclease-free Water | 1 ml | 5 ml | 3×5 ml |
2×TransStart® GoldPfu PCR SuperMix (-dye)
Referência AS401-03
Tamanho : 15×1ml
Marca : TransGen Biotech
2×TransStart® GoldPfu PCR SuperMix (-dye)
Catalog Number: AS401-01
Price:Please inquire first
Product Details
This product is a ready-to-use mixture of TransStart® GoldPfu DNA polymerase, dNTPs, and optimized reaction buffer showing high amplification efficiency, fast amplification, ultra high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water. The amplified products with blunt ends can be directly cloned into pEASY®-Blunt vectors. The SuperMix without dye (-dye) cannot be directly loaded on agarose gel for electrophoresis.
Its PCR product is not suitable for polyacrylamide gel electrophoresis.
Reduced PCR operation time.
Avoid contamination due to multi-step operation.
54-fold fidelity as compared to EasyTaq® DNA Polymerase.
Amplification of genomic DNA fragment up to 15 kb.
Amplification of plasmid DNA fragment up to 20 kb.
Highlights
Fast amplification, ultra high fidelity, high specificity, high stability
Applications
Ultra High fidelity PCR
Site-directed mutagenesis
Blunt end cloning
Complex templates
GC/ AT rich templates
Long fragment PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)