The 5-Hydroxytryptamine (5-HT) BioAssay™ ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 5-HT in serum and plasma.||Detection Range:|11.11-900ng/ml||Sensitivity:|3.97ng/ml||Intra-Assay CV:|<10% ||Inter-Assay CV:|<12%||Kit Components:|*023037A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use|*023037B: Standard, 2x1 vial|023037C: Standard Diluent, 1x20ml|*023037D: Detection Reagent A (green), 1x1vial|*023037E: Detection Reagent B (red), 1x120ul|023037F: Assay Diluent A, 1x12ml|023037G: Assay Diluent B, 1x12ml|023037H: TMB Substrate, 1x9ml|*023037J: Positive Control, 1x1vial, xxng/ml (Lyophilized)|023037L: Wash Buffer, 30X, 1x20ml|023037M: Reagent Diluent, 1x300ul||Storage and Stability:|Store *023037A, *023037B, *023037D, *023037E and *023037J at -20°C. After reconstitution of *023037J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards;|2. Add 50ul standard or sample to each well.|3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediately.