Acetyl-CoA Carboxylase (ACC) Activity Assay Kit
Referência E-BC-K508-M-96T
Tamanho : 96T
Marca : Elabscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Detection Principle | ACC catalyzes acetyl-coA, NaHCO3 and ATP to produce inorganic phosphorus, the activity of ACC can be calculated by measuring the change of inorganic phosphorus content. |
| Synonyms | ACC |
| Sample Type | Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6.78 U/L |
| Detection Range | 6.78-138 U/L |
| Precision | inter-assay CV: 0.08 | intra-assay CV: 0.04 |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Potato tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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