Acidic CK Rat Monoclonal Antibody [Clone ID: OTI9H3]

CAT#: CF190248

Carrier-free (glycerol/BSA-free) Acidic CK rat monoclonal antibody, clone OTI9H3 (formerly 9H3)

Formulation: Standard Carrier-Free


Specifications

Product Data
Clone Name OTI9H3
Applications IHC
Recommended Dilution IHC 1:150
*If antigen retrieval is mandatory, then "Citrate pH6.0 for 10 minutes" can be used
Reactivities Human
Host Rat
Isotype IgG
Clonality Monoclonal
Immunogen Synthetic peptide corresponding to the conserved region of human type I (Acidic) keratins (including KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20).
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Reference Data

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