AMV Reverse Transcriptase (20 u/µL)
Referência P0071-CS
Tamanho : 1,000U
Marca : Canvax Biotech
Contactar o distribuidor local :
Telefone : +1 850 650 7790
AMV Reverse Transcriptase
From Avian Myeloblastosis Virus, purified from E.coli
AMV Reverse Transcriptase, encoded by Avian Myeloblastosis Virus (AMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template.
AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. The enzyme possesses an intrinsic RNase H activity. AMV RT possesses multiple enzymatic activities including RNA- and DNA-directed DNA polymerase, DNA-RNA unwinding activity, a sequence- specific Mn2+ -dependent endonuclease and ribonuclease H.
Source: purified from E.coli strain harboring a plasmid that directs the synthesis of modified form of AMLV-RT.
Detailed information:
- Concentration: 10 U/µL
- Full Solution: includes Reaction Buffer 5x.
- High quality: free of RNase, and DNase (exo- and endonuclease) activities.
Unit definition:
One unit is the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37 °C, using poly(A) oligo dT as a template primer.
– AMV Reverse Transcriptase (10 U/μL)
– Reaction Buffer 5x
Datasheet MSDS- RT PCR.
- Synthesis of cDNA.
- RNA Sequencing.
- Exempt of nucleases (endo, exo and ribonucleases) activities.
- Purity: >90% as judged by SDS-polyacrylamide gels with blue staining.
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.