RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3. Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM166 at 0.5 µg/mL, showed a band of histone H3 acetylated at Lysine 18 in treated HeLa. Sandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 µg/mL) as the capture antibody and biotinylated anti-H3K18C (RM166, 2 ug/mL) as the detection antibody. ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K18Ac antibody (RM166, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys18) Rabbit mAb RM166 (red). Actin filaments have been labeled with fluorescein phalloidin (green). Immunohistochemistry staining of HepG2 cells using Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.
Product Details Product Name Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166 Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation) Catalog No. 31-1055-00 Clone Name RM166 Product Data Sheet Anti-Acetyl-Histone H3 Lys18 Rabbit Monoclonal Antibody Data Sheet RM166 Specificity RM166 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3. Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys18). Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 0.5 µg/mL - 2 µg/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ChIP: 2 µg/mL-10 µg/mL;
IHC: 1 µg/mL-10 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL. Keywords References for Acetyl-Histone H3 (Lys18) antibody [RM166] Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
Product Images
Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166]
![Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1055-00-S
Tamanho : 25ug
Marca : RevMab Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Anti-Acetyl-Histone H3 (Lys18) rabbit monoclonal antibody [RM166]
Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation)
25 µg Trial Size, 100 µg Full Size