Anti-CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I) Monoclonal Antibody

CD44 Iis a a 100kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.||Applications:|Suitable for use in Flow Cytometry, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul|Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment with citrate buffer pH 6.0 prior to staining of paraffin sections.|Immunohistochemistry: Frozen sections|Optimal dilutions to be determined by the researcher. ||Recommended Secondary Antibodies:|I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo|I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo|I1904-14C: IgG, Fc (HRP) Pab Gt xMo|I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.