Lyophilized. 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% (w/v) BSA, Protease/IgG free, 0.05% (w/v) Sodium Azide.
Clonality
Polyclonal
Host
Rabbit
Conjugation
TRITC: Tetramethylrhodamine
Application
IEM, FC
Additional Information
Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Reconstitution and Storage
Reconstitution: Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Storage: 2-8C Shelf Life: Expires 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Purification
Affinity purified using solid phase Hamster IgG
Concentration
1.0 mg/ml (E 1% at 280 nm = 13.0)
Preservative
0.05% (w/v) Sodium Azide
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Application Info
This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s):1:20 - 1:2,000 for most applications