Western Blot analysis of cell lysates prepared from 293T transfected with a DNA construct encoding wild type or K36M mutant proteins of Histone H3.1 and H3.3, using anti-Histone H3 K36M, Clone RM193. Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3 K36M, stained with anti-Histone H3 K36M, Clone RM193. Immunohistochemical staining of formalin fixed paraffin embedded (FFPE) Chondroblastoma tumor cell tissue sections using RM193 Anti-Histone H3 K36M mAb at a 1:400 dilution. Image courtesy of Adrienne Flanagan, Department of Histopathology, RONH, Stanmore, Middlesex, UK
Product Details Product Name Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193 Product Description Rabbit monoclonal to Histone H3 K36M; Histone H3, K36M Mutant Catalog No. 31-1085-00-S;
31-1085-00-L;
31-1085-02 Clone Name RM193 Product Data Sheet Anti-Histone H3 K36M Rabbit Monoclonal Antibody Data Sheet RM193
Biotinylated Anti-Histone H3 K36M Rabbit Monoclonal Antibody Data Sheet RM193 Specificity RM193 reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3. Immunogen A peptide corresponding to Histone H3 K36M mutant Application Western Blot
ELISA
Immunohistochemistry
Chromatin IP
Species Reactivity All Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage WB: 1:200-1:1000;
ELISA: 1:250-1:2500;
IHC: 1:100-1:500. Keywords H3F3A ; H3F3B ; K36M; bone tumour; chondroblastoma References for Histone H3 K36M Mutant antibody [RM193] Papke D J et al. Clinicopathologic characterization of malignant chondroblastoma: a neoplasm with locally aggressive behavior and metastatic potential that closely mimics chondroblastoma-like osteosarcoma. Mod Pathol. (2020), 10.1038/s41379-020-0604-2. IHC; Human. Read more (PubMed: 32601382)
Brumbaugh J et al. Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo. Nat Cell Biol. (2019), 10.1038/s41556-019-0403-5. WB; Human. Read more (PubMed: 31659274)
Lu C et al. Histone H3K36M mutation and trimethylation patterns in chondroblastoma. Histopathology. (2019), 10.1111/his.13725. IHC; Human. Read more (PubMed: 30098026)
Amary F et al. Synovial chondromatosis and soft tissue chondroma: extraosseous cartilaginous tumor defined by FN1 gene rearrangement. Mod Pathol. (2019), 10.1038/s41379-019-0315-8. IHC; Human. Read more (PubMed: 31273315)
Fang D et al. Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations. Methods Mol Biol. (2018), 10.1007/978-1-4939-8663-7_19. IHC-P; Human. Read more (PubMed: 30073537)
Frye J et al. H3F3 K36M Immunohistochemistry is a Useful Diagnostic Marker for Chondroblastoma in Small Biopsy Specimens. Mod Pathol. (2018), USCAP 2018 Abstracts-Bone & Soft Tissue Pathology (45-120). IHC-P; Human. Read more (USCAP 2018 Abstracts: 63: PubMed: 29551782)
Amary MF et al. The H3F3 K36M mutant antibody is a sensitive and specific marker for the diagnosis of chondroblastoma. Histopathology (2016) 10.1111/his.12945. IHC-P; Human. Read more (PubMed: 26844533)
Fang D et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas. Science (2016) 10.1126/science.aae0065. ChIP; Human. Read more (PubMed: 27229140)
Jundt G et al. Chondroblastoma. Der Pathologe (2018) 10.1007/s00292-017-0397-3. IHC-P; Human Read more (PubMed: 29209799)
Schaefer IM et al. Immunohistochemistry for histone H3G34W and H3K36M is highly specific for giant cell tumor of bone and chondroblastoma, respectively, in FNA and core needle biopsy. Cancer Cytopathol. (2018) 10.1002/cncy.22000. IHC-P; Human. Read more (PubMed: 29757500)
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Anti-Histone H3 K36M rabbit monoclonal antibody [RM193]
![Anti-Histone H3 K36M rabbit monoclonal antibody [RM193]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1085-00-S
Tamanho : 100ul
Marca : RevMab Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Rabbit monoclonal to Histone H3 K36M;
Histone H3, K36M Mutant
100 µL Small Size, 400 µL Large Size, 50 µL Biotin Conjugate
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