RM140 specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3. Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM140 at 0.5 µg/mL, showed a band of histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells. ChIP performed on HeLa cells using H3K4me1 antibody (RM140, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. Immunocytochemistry of HeLa cells treated with sodium butyrate, using Monomethyl-Histone H3 (Lys4) Rabbit mAb RM140 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Product Details Product Name Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140 Product Description Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4) Catalog No. 31-1046-00 Clone Name RM140 Product Data Sheet Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM140 Specificity RM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3 Immunogen A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4) Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 0.2 µg/mL – 1 µg/mL;
ChIP: 2 µg/mL- 10 µg/mL;
ICC: 1 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL. Keywords . References Shah RN et al Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell. (2018), 10.1016/j.molcel.2018.08.015. ICeChIP. Read more (PubMed: 30244833) Product Images
Anti-Monomethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM140]
![Anti-Monomethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM140]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1046-00-S
Tamanho : 25ug
Marca : RevMab Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Anti-Monomethyl-Histone H3 (Lys4) rabbit monoclonal antibody [RM140]
Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4)
25 µg Trial Size, 100 µg Full Size
