Anti-RFP (CHICKEN) Antibody

Referência 600-901-379

Tamanho : 100ug

Marca : Rockland Immunochemicals

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Specifications for RFP Antibody

Product Details

Anti-RFP (CHICKEN) Antibody - 600-901-379
chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Chicken
Polyclonal
IgY

Target Details

DsRed  - View All DsRed Products
RFP, rRFP, tdTomato
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by Western blot.
Q9U6Y8 - UniProtKB

Application Details

ELISA, WB
FC, IF, IHC, Multiplex  - View References
Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA and Western blot to detect RFP. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by Western blot.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Background

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays such as Western blotting.

References (62)

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IHC, ICC, Histology
PubMed
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IF, Confocal Microscopy
PubMed
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IF, Confocal Microscopy
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FC, FACS, FLOW; IF, Confocal Microscopy
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IF, Confocal Microscopy
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IHC, ICC, Histology
PubMed
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IF, Confocal Microscopy
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IHC, ICC, Histology
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IHC, ICC, Histology
PubMed
(). Transcriptional profiling of sequentially generated septal neuron fates. Elife.
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IF, Confocal Microscopy
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IF, Confocal Microscopy
PubMed
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IF, Confocal Microscopy; IHC, ICC, Histology
PubMed
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IF, Confocal Microscopy
PubMed
(). Smooth muscle cell reprogramming in aortic aneurysms. Cell Stem Cell.
Applications
FC, FACS, FLOW; IF, Confocal Microscopy
PubMed
(). Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes. Development.
Applications
IHC, ICC, Histology
PubMed
(). Prdm1 overexpression causes a photoreceptor fate-shift in nascent, but not mature, bipolar cells. Dev Biol.
Applications
IHC, ICC, Histology
PubMed
(). Single-cell transcriptomic analyses of the developing meninges reveal meningeal fibroblast diversity and function. Dev Cell.
Applications
IF, Confocal Microscopy
PubMed
(). Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges. Acta Neuropathol.
Applications
IHC, ICC, Histology
PubMed
(). Cystatin B is essential for proliferation and interneuron migration in individuals with EPM1 epilepsy. EMBO Mol Med.
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IHC, ICC, Histology
PubMed
(). Modeling Human-specific Interlaminar Astrocytes in the Mouse Cerebral Cortex. J Comp Neurol.
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IHC, ICC, Histology
PubMed
(). Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol.
Applications
IF, Confocal Microscopy
PubMed
(). The ELAV/Hu protein Found in neurons regulates cytoskeletal and ECM adhesion inputs for space-filling dendrite growth. PLoS Genet.
Applications
IF, Confocal Microscopy
PubMed
(). Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates. Nature Communications
Applications
IF, Confocal Microscopy
PubMed
(). GABAergic interneurons excite neonatal hippocampus in vivo. Science Advances
Applications
IHC, ICC, Histology
PubMed
(). A Feasibility Study to Investigate Chemogenetic Modulation of the Locus Coeruleus by Means of Single Unit Activity. Frontiers in Neuroscience
Applications
IHC, ICC, Histology
PubMed
(). Methods for renal lineage tracing: In vivo and beyond. Methods Cell Biol.
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IF, Confocal Microscopy
PubMed
(). Engram Cell Excitability State Determines the Efficacy of Memory Retrieval. Neuron.
Applications
IHC, ICC, Histology
PubMed
(). Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse. J Anat.
Applications
IHC, ICC, Histology
PubMed
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Applications
IF, Confocal Microscopy
PubMed
(). Diabetes-Induced NF-κB Dysregulation in Skeletal Stem Cells Prevents Resolution of Inflammation. Diabetes.
Applications
IF, Confocal Microscopy
PubMed
(). Dissection of central clock function in Drosophila through cell-specific CRISPR-mediated clock gene disruption. Elife.
Applications
IHC, ICC, Histology
PubMed
(). Chronic nigral neuromodulation aggravates behavioral deficits and synaptic changes in an α-synuclein based rat model for Parkinson's disease. Acta Neuropathologica Communications
Applications
IHC, ICC, Histology
PubMed
(). A cell fitness selection model for neuronal survival during development. Nat Commun.
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). Cell-Type-Specific Role of ΔFosB in Nucleus Accumbens In Modulating Intermale Aggression. The Journal of Neuroscience
Applications
IF, Confocal Microscopy
PubMed
(). Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes. Nature Neuroscience
Applications
IHC, ICC, Histology
PubMed
(). Unique morphogenetic signatures define mammalian neck muscles and associated connective tissues. Elife
Applications
IF, Confocal Microscopy
PubMed
(). Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor. Nature.
Applications
IHC, ICC, Histology
PubMed
(). Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. Neuron.
Applications
IF, Confocal Microscopy
PubMed
(). Sodium Channel SCN3A (NaV1.3) Regulation of Human Cerebral Cortical Folding and Oral Motor Development. Neuron
Applications
IHC, ICC, Histology
PubMed
(). Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development. Elife
Applications
IF, Confocal Microscopy
PubMed
(). Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy. Cell Stem Cell
Applications
IF, Confocal Microscopy
PubMed
(). Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity. Cell
Applications
IF, Confocal Microscopy
PubMed
(). Specialized Mechanosensory Nociceptors Mediating Rapid Responses to Hair Pull. Neuron.
Applications
IF, Confocal Microscopy
PubMed
(). Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia. Journal of Clinical Investigation
Applications
WB, IB, PCA
PubMed
(). A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research. G3: Genes, Genomes, Genetics
Applications
IHC, ICC, Histology
PubMed
(). The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation. Nature Communications
Applications
IHC, ICC, Histology
PubMed
(). Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes. PloS Genetics
Applications
WB, IB, PCA
PubMed
(). Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity. Autophagy
Applications
WB, IB, PCA
PubMed
(). Regulated delivery of molecular cargo to invasive tumour-derived microvesicles. Nature Communications
Applications
WB, IB, PCA
PubMed

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Flow Cytometry (FC) Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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