Anti-STREPTAVIDIN Antibody
Referência OARA01739
Tamanho : 2mL
Marca : Aviva Systems Biology
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Anti-STREPTAVIDIN Antibody (OARA01739)
| Datasheets/Manuals | Printable datasheet for Anti-STREPTAVIDIN Antibody (OARA01739) |
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| Predicted Species Reactivity | Streptavidin |
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| Product Format | Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | DB|ELISA|IF|IHC|WB |
| Additional Information | Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| :: | Preservative: 0.01% (w/v) Sodium Azide Stabilizer: None |
| Reconstitution and Storage | -20°C or -80°C|2°C to 8°C |
| Immunogen | Streptavidin (Streptomyces avidinii) |
| Purification | Anti-STREPTAVIDIN antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Streptavidin. |
| Predicted Homology Based on Immunogen Sequence | Streptomyces avidinii |
| Concentration | 70 mg/ml |
| Specificity | Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. No cross reactivity occurs against Avidin. |
| Application Info | Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. ELISA: 1:20,000-1:100,000 WB: 1:2,000-1:10,000 IHC: 1:1,000-1:5,000 |
| Description of Target | Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^-14 mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH. |
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