The ApexPrep DNA Plasmid Miniprep kit is based on alkaline lysis technology, which can lyse cells and then adsorb DNA to the adsorption membrane in the presence of high salt. Subsequent steps such as washing away impurities can efficiently and quickly extract up to 30 μg of plasmid DNA. This kit uses a unique buffer formula to maximize the removal of protein impurities and other organic compounds, and can process 1-5 mL of bacterial solution each time. The yield and quality of plasmid extraction are related to the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics, and other factors.

Plasmid DNA prepared by ApexPrep DNA Plasmid Miniprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and trans-formation, in vitro translation, and transfection of robust cells.