Images
Immunofluorescence
Note

Application Data with Unconjugated Antibody

Immunofluorescence

Immunofluorescence staining of Aqp4 in rat kidney with Aqp4 polyclonal antibody (Cat # PAB28892) at 1:200 dilution, DAPI merged.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against recombinant Rat Aqp4.

    Immunogen

    Recombinant protein corresponding to Rat Aqp4 C-terminus.

    Sequence

    CTKGSYMEVEDNRSQVETED

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Specificity

    0.5 ug/mL of PAB28894 was sufficient for detection of Aqp4, ~35kDa and sometimes larger glycosylated bands ~50kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Form

    Liquid

    Conjugation

    ATTO 488

    Purification

    Affinity purification

    Recommend Usage

    Immunofluorescence (1:400)
    Western Blot (1:1000-4000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    PBS pH7.4, 50% glycerol and 0.09% sodium azide

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody

    Immunofluorescence

    Immunofluorescence staining of Aqp4 in rat kidney with Aqp4 polyclonal antibody (Cat # PAB28892) at 1:200 dilution, DAPI merged.

  • Applications

    Western Blot

    Immunofluorescence

  • Gene Info — Aqp4

    Entrez GeneID

    25293

    Protein Accession#

    NP_036957.1

    Gene Name

    Aqp4

    Gene Alias

    AQP-4, AQP4.M23, MIWC2, Miwc

    Gene Description

    aquaporin 4

    Gene Ontology

    Hyperlink

    Other Designations

    aquaporin 4.M23|mercurial-insensitive water channel