ATP4A (NM_000704) Human Tagged ORF Clone
Referência RC211772
Tamanho : 10ug
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ATP4A (NM_000704) Human Tagged ORF Clone
SKU
RC211772
ATP4A (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)
- TrueORF Gold
Protein expression verified by Western blot, fully sequenced and in stock
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Bulk Requests & Clone Modifications
| Product Data | |
| Target Symbol | ATP4A |
|---|---|
| Synonyms | ATP6A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC211772 representing NM_000704 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGCCGAGAACTATGAGCTCTACTCGGTGGAGCTGGGTCCTGGCCCTGGCGGGGACATGGCTG CCAAGATGAGCAAGAAGAAGAAGGCGGGTGGCGGGGGTGGCAAGAGGAAGGAGAAGCTGGAGAACATGAA GAAGGAGATGGAGATTAACGACCACCAGCTGTCAGTGGCGGAGCTGGAACAGAAATACCAGACCAGTGCC ACCAAGGGCCTCTCTGCGAGCCTGGCTGCTGAGCTGCTGCTGCGGGATGGGCCCAACGCACTGCGGCCAC CACGGGGCACCCCAGAGTACGTCAAGTTTGCGAGGCAGCTGGCCGGGGGCCTGCAGTGCCTCATGTGGGT TGCCGCCGCCATCTGCCTCATCGCCTTTGCCATCCAGGCTAGTGAGGGGGACCTCACCACCGACGACAAT CTGTACCTGGCAATCGCTCTCATTGCTGTGGTTGTCGTCACCGGCTGCTTTGGCTACTACCAGGAATTCA AGAGCACCAACATCATCGCCAGCTTTAAGAACCTTGTGCCACAGCAAGCCACTGTCATCCGCGATGGAGA CAAATTCCAGATCAACGCTGACCAACTGGTGGTGGGCGACCTGGTGGAGATGAAAGGTGGGGACAGAGTG CCCGCCGACATCCGCATCCTGGCGGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTGACAGGGGAGTCTG AGCCACAGACCCGCTCACCCGAGTGCACGCACGAGAGCCCTCTGGAGACCCGCAACATCGCCTTCTTCTC CACCATGTGCCTTGAGGGCACCGTGCAGGGCCTGGTGGTGAACACGGGCGACCGCACCATCATTGGGCGC ATCGCATCGCTGGCGTCGGGGGTGGAAAACGAGAAGACACCCATCGCTATCGAGATCGAGCATTTTGTGG ACATCATCGCGGGCCTGGCCATTCTCTTCGGTGCCACATTTTTTATTGTGGCCATGTGCATTGGCTACAC CTTCCTGCGGGCCATGGTCTTCTTCATGGCCATCGTGGTGGCCTATGTGCCTGAGGGGCTGCTGGCCACT GTCACAGTCTGCCTGTCCCTGACAGCCAAGCGCCTGGCCAGTAAGAACTGCGTGGTCAAGAACCTGGAGG CGGTGGAGACATTGGGCTCCACTTCGGTGATCTGCTCGGACAAGACAGGGACTCTCACTCAGAACCGCAT GACTGTGTCCCATCTGTGGTTTGACAACCACATCCACACAGCTGACACCACGGAAGACCAGTCAGGGCAG ACGTTTGACCAGTCCTCGGAGACGTGGCGGGCGCTGTGCCGGGTGCTCACCCTGTGCAACCGCGCCGCCT TCAAGTCCGGCCAGGATGCAGTGCCTGTGCCCAAGCGCATCGTGATTGGAGACGCATCGGAGACGGCGCT GCTCAAGTTCTCGGAGCTGACGCTGGGCAACGCCATGGGCTACCGGGACCGCTTCCCAAAAGTCTGCGAG ATACCCTTCAACTCCACCAACAAGTTCCAGCTGTCCATCCATACGCTGGAGGACCCGCGGGACCCGCGAC ACTTGCTGGTGATGAAGGGCGCCCCCGAGCGCGTGCTGGAGCGCTGCAGCTCCATCCTTATCAAGGGCCA GGAGCTGCCGCTGGACGAGCAGTGGCGCGAGGCCTTCCAGACCGCCTACCTCAGCCTGGGAGGCCTGGGC GAACGCGTGCTCGGCTTCTGCCAGCTCTACCTGAATGAGAAGGACTACCCGCCTGGCTATGCCTTCGACG TAGAGGCCATGAACTTTCCATCTAGCGGCCTCTGCTTTGCGGGACTTGTATCCATGATTGACCCACCCCG GGCCACCGTCCCTGATGCTGTGCTCAAGTGTCGCACCGCAGGCATCCGGGTGATCATGGTAACGGGTGAC CACCCCATCACCGCCAAGGCCATTGCAGCCAGTGTGGGCATCATCTCGGAAGGCAGCGAGACAGTGGAGG ACATCGCTGCCCGCCTCCGTGTGCCCGTAGACCAGGTTAATCGCAAGGATGCCCGTGCCTGTGTGATCAA TGGCATGCAGCTGAAGGACATGGACCCATCGGAACTGGTCGAGGCCCTGCGCACCCACCCCGAGATGGTG TTTGCGCGCACCAGCCCCCAGCAGAAGCTGGTGATCGTGGAGAGCTGCCAGCGGCTGGGTGCGATTGTGG CCGTCACGGGGGATGGTGTGAATGACTCCCCAGCTCTGAAGAAGGCAGACATCGGAGTAGCCATGGGCAT CGCTGGCTCAGATGCTGCCAAAAATGCAGCTGACATGATCCTGCTGGATGACAACTTTGCCTCCATTGTG ACAGGCGTGGAGCAGGGTCGACTGATCTTCGACAACCTGAAGAAGTCTATTGCCTACACATTGACCAAGA ACATCCCAGAGCTGACACCCTACCTCATCTACATCACCGTCAGCGTGCCCCTGCCCCTCGGGTGCATCAC CATCCTCTTCATCGAACTCTGCACTGACATTTTCCCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGT GACATCATGCACCTGCGTCCACGCAACCCAAAGCGTGACAGATTGGTCAACGAGCCCCTGGCTGCCTACT CCTACTTCCAGATTGGTGCCATTCAGTCCTTTGCTGGCTTCACTGACTACTTCACGGCAATGGCCCAGGA GGGCTGGTTCCCACTGCTGTGCGTGGGGCTGCGGGCGCAGTGGGAGGACCACCACCTACAAGATCTGCAG GACAGCTACGGCCAGGAGTGGACATTCGGGCAGCGCCTGTACCAGCAGTACACCTGCTACACCGTGTTCT TCATCAGCATTGAGGTGTGCCAGATCGCCGATGTCCTCATCCGCAAGACGCGCCGTCTCTCTGCCTTCCA GCAAGGCTTCTTCAGGAATAAGATCCTGGTGATCGCCATCGTGTTCCAGGTCTGCATCGGCTGCTTCCTG TGCTACTGCCCCGGCATGCCCAACATCTTCAACTTCATGCCCATTCGGTTCCAGTGGTGGCTGGTCCCCC TGCCCTACGGCATCCTCATCTTCGTCTATGATGAGATCCGGAAGCTTGGAGTTCGCTGTTGCCCAGGGAG CTGGTGGGACCAGGAACTCTACTATAGCGGACCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC211772 representing NM_000704 Red=Cloning site Green=Tags(s) MGKAENYELYSVELGPGPGGDMAAKMSKKKKAGGGGGKRKEKLENMKKEMEINDHQLSVAELEQKYQTSA TKGLSASLAAELLLRDGPNALRPPRGTPEYVKFARQLAGGLQCLMWVAAAICLIAFAIQASEGDLTTDDN LYLAIALIAVVVVTGCFGYYQEFKSTNIIASFKNLVPQQATVIRDGDKFQINADQLVVGDLVEMKGGDRV PADIRILAAQGCKVDNSSLTGESEPQTRSPECTHESPLETRNIAFFSTMCLEGTVQGLVVNTGDRTIIGR IASLASGVENEKTPIAIEIEHFVDIIAGLAILFGATFFIVAMCIGYTFLRAMVFFMAIVVAYVPEGLLAT VTVCLSLTAKRLASKNCVVKNLEAVETLGSTSVICSDKTGTLTQNRMTVSHLWFDNHIHTADTTEDQSGQ TFDQSSETWRALCRVLTLCNRAAFKSGQDAVPVPKRIVIGDASETALLKFSELTLGNAMGYRDRFPKVCE IPFNSTNKFQLSIHTLEDPRDPRHLLVMKGAPERVLERCSSILIKGQELPLDEQWREAFQTAYLSLGGLG ERVLGFCQLYLNEKDYPPGYAFDVEAMNFPSSGLCFAGLVSMIDPPRATVPDAVLKCRTAGIRVIMVTGD HPITAKAIAASVGIISEGSETVEDIAARLRVPVDQVNRKDARACVINGMQLKDMDPSELVEALRTHPEMV FARTSPQQKLVIVESCQRLGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDAAKNAADMILLDDNFASIV TGVEQGRLIFDNLKKSIAYTLTKNIPELTPYLIYITVSVPLPLGCITILFIELCTDIFPSVSLAYEKAES DIMHLRPRNPKRDRLVNEPLAAYSYFQIGAIQSFAGFTDYFTAMAQEGWFPLLCVGLRAQWEDHHLQDLQ DSYGQEWTFGQRLYQQYTCYTVFFISIEVCQIADVLIRKTRRLSAFQQGFFRNKILVIAIVFQVCIGCFL CYCPGMPNIFNFMPIRFQWWLVPLPYGILIFVYDEIRKLGVRCCPGSWWDQELYYSGP SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_000704 |
| ORF Size | 3114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000704.2, NP_000695.2 |
| RefSeq Size | 3582 bp |
| RefSeq ORF | 3108 bp |
| Locus ID | 495 |
| UniProt ID | P20648 |
| Cytogenetics | 19q13.12 |
| MW | 113.9 kDa |
| Summary | The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC211772L1 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged | 10 ug | | |
| RC211772L2 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged | 10 ug | | |
| RC211772L3 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged | 10 ug | | |
| RC211772L4 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged | 10 ug | | |
| RG211772 | ATP4A (tGFP-tagged) - Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) | 10 ug | | |
| SC300122 | ATP4A (untagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.