B1-8 Human IgG4-S228P Isotype Control, In Vivo Grade Recombinant Human

The anti-NP B1-8 antibody (IgG2c, kappa) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The recombinant B1-8 IgG2c kappa isotype control and mutants (natural and engineered mIgG2c isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does express the mouse IgG2a antibody, and vice versa. If one uses any of the mouse strains for animal research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies. There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. Thus, the wild type mouse IgG2c isotype control and the Fc silenced mouse IgG2c isotype control mutants should be used in such an application.||The in vivo grade recombinant B1-8 mouse IgG2c isotype control antibody (mIgG2c isotype control) was produced in mammalian cells.||Recombinant anti-NP isotype control antibodies and Fc silenced mutants from clone B1-8 good for in vitro and in vivo studies. Low or no specific binding to human, rat, and mouse samples tested.||Applications: |Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and in vivo animal model research. Other applications not tested.||Recommended Dilution:|Immunohistochemistry: frozen/paraffin|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.