Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG3 antibody, Clone RM119. Sandwich ELISA using RM119 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin. Sandwich ELISA using RM119 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin. ELISA of human immunoglobulins shows RM119 reacts only to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Product Details Product Name Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119 Product Description Biotinylated Rabbit monoclonal to Human IgG3 Catalog No. 31-1021-02 Clone Name RM119 Product Data Sheet Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119 Specificity RM119 reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. Immunogen Human IgG3 Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL. Keywords . Product Images
Biotin Anti-Human IgG3 rabbit monoclonal antibody [RM119]
![Biotin Anti-Human IgG3 rabbit monoclonal antibody [RM119]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1021-02
Tamanho : 50ug
Marca : RevMab Biosciences
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Biotinylated Rabbit monoclonal to Human IgG3
50 µg
