Western blot of nonreduced (-) and reduced (+) mouse IgG1κ and IgG1λ (20 ng/lane), using 0.2 . This antibody reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa). ELISA of mouse immunoglobulins shows RM103 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. Analkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Product Details Product Name Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103 Product Description Biotinylated Rabbit monoclonal to Mouse Ig Kappa Light Chain Catalog No. 31-1007-02 Clone Name RM103 Product Data Sheet Biotin Anti-Mouse Ig Kappa Rabbit Monoclonal Antibody Data Sheet RM103 Specificity This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM103 has been engineered to eliminate Fc receptor binding. Immunogen Mouse IgG Application ELISA
Western Blot (WB) (nonreduced) Species Reactivity Mouse Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0"> Stability Stable for 1 Year at -20.0"> Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 "> Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin"> Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.005 "> Keywords . Product Images 
Biotin Anti-Mouse Ig Kappa Light Chain rabbit monoclonal antibody [RM103]
![Biotin Anti-Mouse Ig Kappa Light Chain rabbit monoclonal antibody [RM103]](https://www.quimigen.pt/upload/-img-lapin1.png)
Referência 31-1007-02
Tamanho : 50ug
Marca : RevMab Biosciences
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Telefone : +1 850 650 7790
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![Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [Clone RM103], 31-1007-02 - Image 2](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNC8wMS9BbnRpLW1vdXNlLWthcHBhMi5qcGc=.jpg)
![Biotin Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody [Clone RM103], 31-1007-02 - Image 3](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNC8wMS9BbnRpLW1vdXNlLWthcHBhMy5qcGc=.jpg)
Biotinylated Rabbit monoclonal to Mouse Ig Kappa Light Chain
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