Purified recombinant protein of Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant G, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
Specifications
Product Data
Species
Human
Expression Host
E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
A DNA sequence encoding human full-length CASP8
Tag
N-His
Predicted MW
61.7 kDa
Concentration
>0.05 µg/µL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer
25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Note
For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage
Store at -80°C.
Stability
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Transient overexpression lysate of caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant A
100 ug
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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