Caspofungin (MK-0991) diacetate is a potent antifungal agent. Caspofungin diacetate inhibits the synthesis of the fungal cell wall component β-(l,3)-D-glucan.
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Caspofungin diacetate Chemical Structure
CAS No. :
179463-17-3
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Based on 6 publication(s) in Google Scholar
Other Forms of Caspofungin diacetate:
Caspofungin-d4 diacetate
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Caspofungin
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Caspofungin (diacetate) (Standard)
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Caspofungin diacetate purchased from MedChemExpress. Usage Cited in:
Cell Physiol Biochem. 2016 Aug 12;39(3):939-949.
[Abstract]
Arithmetic means±SEM (n=21) of erythrocyte annexin-V-binding following incubation for 48 hours to Ringer solution without (white bar) or with (black bars) Caspofungin (30-60 µg/mL).
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Caspofungin (MK-0991) diacetate is a potent antifungal agent. Caspofungin diacetate inhibits the synthesis of the fungal cell wall component β-(l,3)-D-glucan[1][2].
In Vivo
Caspofungin diacetate (1-8 mg/kg; i.p.; daily, for 7 days) is able to penetrate the CNS in mice and achieve concentrations that result in the reduction of Candida burden in the brain[1].
Caspofungin diacetate (0.41-41 μM; i.p.; for 5 weeks; male C57BL/6 mice) is a safe antifungal agent at vitreal concentrations of 0.41 to 4.1 μM in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Complement component 5-deficient DBA/2N mice[1]
Dosage:
1, 2, 4 and 8 mg/kg
Administration:
Intraperitoneal injection; daily, for 7 days
Result:
Reduced the concentration of Candida load in the brain.
Animal Model:
Male C57BL/6 mice[2]
Dosage:
0.41, 1.2, 2.5, 4.1, and 41 μM
Administration:
Intraperitoneal injection; for 5 weeks
Result:
Had nonsignificant alterations in their ERG waveforms from 0.41 to 4.1 μM.
Room temperature in continental US; may vary elsewhere.
Stockage
-20°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
In Vitro:
H2O : ≥ 100 mg/mL (82.41 mM)
DMSO : 100 mg/mL (82.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
*"≥" means soluble, but saturation unknown.
Preparing Stock Solutions
ConcentrationSolventMass
1 mg
5 mg
10 mg
1 mM
0.8241 mL
4.1206 mL
8.2412 mL
5 mM
0.1648 mL
0.8241 mL
1.6482 mL
10 mM
0.0824 mL
0.4121 mL
0.8241 mL
View the Complete Stock Solution Preparation Table
*Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
*
Note: If you choose water as the stock solution, please dilute it to the working solution,
then filter and sterilize it with a 0.22 μm filter before use.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Protocol 1
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3
Add each solvent one by one: 10% DMSO 90% Corn Oil
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μLDMSO stock solution (20.8 mg/mL) to 900 μLCorn oil, and mix evenly.
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution.
If precipitation or phase separation occurs during preparation,
heat and/or sonication can be used to aid dissolution.
Protocol 1
Add each solvent one by one: PBS
Solubility: 100 mg/mL (82.41 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage
mg/kg
Animal weight (per animal)
g
Dosing volume (per animal)
μL
Number of animals
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
[1]. Flattery AM, et, al. Efficacy of caspofungin in a juvenile mouse model of central nervous system candidiasis. Antimicrob Agents Chemother. 2011 Jul;55(7):3491-7.
[Content Brief]
[2]. Mojumder DK, et, al. Evaluating retinal toxicity of intravitreal caspofungin in the mouse eye. Invest Ophthalmol Vis Sci. 2010 Nov;51(11):5796-803.
[Content Brief]
[1]. Flattery AM, et, al. Efficacy of caspofungin in a juvenile mouse model of central nervous system candidiasis. Antimicrob Agents Chemother. 2011 Jul;55(7):3491-7.
[2]. Mojumder DK, et, al. Evaluating retinal toxicity of intravitreal caspofungin in the mouse eye. Invest Ophthalmol Vis Sci. 2010 Nov;51(11):5796-803.
Complete Stock Solution Preparation Table
*Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent
ConcentrationSolventMass
1 mg
5 mg
10 mg
25 mg
H2O / DMSO
1 mM
0.8241 mL
4.1206 mL
8.2412 mL
20.6029 mL
5 mM
0.1648 mL
0.8241 mL
1.6482 mL
4.1206 mL
10 mM
0.0824 mL
0.4121 mL
0.8241 mL
2.0603 mL
15 mM
0.0549 mL
0.2747 mL
0.5494 mL
1.3735 mL
20 mM
0.0412 mL
0.2060 mL
0.4121 mL
1.0301 mL
25 mM
0.0330 mL
0.1648 mL
0.3296 mL
0.8241 mL
30 mM
0.0275 mL
0.1374 mL
0.2747 mL
0.6868 mL
40 mM
0.0206 mL
0.1030 mL
0.2060 mL
0.5151 mL
50 mM
0.0165 mL
0.0824 mL
0.1648 mL
0.4121 mL
60 mM
0.0137 mL
0.0687 mL
0.1374 mL
0.3434 mL
80 mM
0.0103 mL
0.0515 mL
0.1030 mL
0.2575 mL
*
Note: If you choose water as the stock solution, please dilute it to the working solution,
then filter and sterilize it with a 0.22 μm filter before use.
Caspofungin diacetate Related Classifications
Anti-infection
FungalAntibioticBacterial
Help & FAQs
Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
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