CD81 ELISA Kit (Mouse)
Referência OKCA02018
Tamanho : 96Wells
Marca : Aviva Systems Biology
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CD81 ELISA Kit (Mouse) (OKCA02018)
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. Please click here to view the MSDS. |
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| COA Datasheet | ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
| Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Cd81 ELISA Kit (Mouse) (OKCA02018) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd81 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd81 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cd81 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 46.88 - 3,000 pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV <12% (n=20) Mean Inter-Assay: CV <15% (n=24) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Lysates | ||||||||||||||||||||||
| Sensitivity | 11.72 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | CD81 |
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| Alias Symbols | Ta, Tap, Tsp, Tapa1, Tapa-1, Tspan28 |
| NCBI Gene Id | 12520 |
| Protein Name | CD81 antigen |
| Description of Target | May play an important role in the regulation of lymphoma cell growth. May be involved in the acrosome reaction. |
| Uniprot ID | P35762 |
| Protein Accession # | NP_598416 |
| Nucleotide Accession # | NM_133655 |
