CHAPS
Referência M19964-500mg
Tamanho : 500mg
Marca : AbMole Bioscience
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Biological Activity
CHAPS is a non-denaturing, zwitterionic detergent that dissolves membrane proteins. CHAPS are used to stabilize various protein-DNA complexes and can preserve the biochemical activity of proteins in solution. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.
Chemical Information
| Molecular Weight | 614.88 |
| Formula | C32H58N2O7S |
| CAS Number | 75621-03-3 |
| Solubility (25°C) | Water ≥ 50 mg/mL |
| Storage | 2-8°C, dry, sealed |
References
[1] P M Rodi, et al. Biochim Biophys Acta . Comparative study of the interaction of CHAPS and Triton X-100 with the erythrocyte membrane
[2] Ana Kroflic, et al. Langmuir. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence