Chloramphenicol [56-75-7]
Referência HY-B0239-1g
Tamanho : 1g
Marca : MedChemExpress
Chloramphenicol is an orally active, potent and broad-spectrum antibiotic. Chloramphenicol shows antibacterial activity. Chloramphenicol represses the oxygen-labile transcription factor and hypoxia inducible factor-1 alpha (HIF-1α) in hypoxic A549 and H1299 cells. Chloramphenicol suppresses the mRNA levels of vascular endothelial growth factor (VEGF) and glucose transporter 1, eventually decreasing VEGF release. Chloramphenicol can be used for anaerobic infections and lung cancer research.
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Chloramphenicol Chemical Structure
CAS No. : 56-75-7
This product is a controlled substance and not for sale in your territory.
Based on 8 publication(s) in Google Scholar
Other Forms of Chloramphenicol:
- Chloramphenicol-d5 In-stock
- Chloramphenicol palmitate In-stock
- Levomecol Obtenir un devis
- DL-threo-Chloramphenicol-d5 Obtenir un devis
- Threo-Chloramphenicol-d6 Obtenir un devis
- Chloramphenicol-d4 Obtenir un devis
Voir tous les produits spécifiques à Isoform Antibiotic:
Voir tous les produits spécifiques à Isoform HIF/HIF Prolyl-Hydroxylase:
Voir tous les produits spécifiques à Isoform VEGFR:
Voir tous les produits spécifiques à Isoform JNK:
Voir tous les produits spécifiques à Isoform Akt:
Voir tous les produits spécifiques à Isoform MMP:
Description |
Chloramphenicol is an orally active, potent and broad-spectrum antibiotic. Chloramphenicol shows antibacterial activity. Chloramphenicol represses the oxygen-labile transcription factor and hypoxia inducible factor-1 alpha (HIF-1α) in hypoxic A549 and H1299 cells. Chloramphenicol suppresses the mRNA levels of vascular endothelial growth factor (VEGF) and glucose transporter 1, eventually decreasing VEGF release. Chloramphenicol can be used for anaerobic infections and lung cancer research[1][2][3]. |
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IC50 & Target |
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In Vitro |
Chloramphenicol (1-100 μg/mL, 18-24 h) inhibits the HIF-1α pathway in NSCLC cells in a concentration-dependent manner[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assay[1]
Western Blot Analysis[1]
Western Blot Analysis[1]
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In Vivo |
Chloramphenicol (0-3500 mg/kg, Gavage, daily, for 5 days) decreases erythrocytes and erythrocyte precursors and reduces marrow erythroid cells were at day 1 post-dosing, and returns to normal by 14 days post-dosing[4]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Masse moléculaire |
323.13 |
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Formule |
C11H12Cl2N2O5 |
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CAS No. |
56-75-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=C(N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1)C(Cl)Cl |
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Structure Classification |
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Initial Source |
Streptomyces venezuelae |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
*The compound is unstable in solutions, freshly prepared is recommended. |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 150 mg/mL (464.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Ethanol : 100 mg/mL (309.47 mM; Need ultrasonic) H2O : 3.06 mg/mL (9.47 mM; Need ultrasonic) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*The compound is unstable in solutions, freshly prepared is recommended.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: 99.82% COA (247 KB) HNMR (165 KB) LCMS (449 KB) Instruction de manipulation (2659 KB) |
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Références |
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Complete Stock Solution Preparation Table
* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / Ethanol / DMSO | 1 mM | 3.0947 mL | 15.4736 mL | 30.9473 mL | 77.3682 mL |
5 mM | 0.6189 mL | 3.0947 mL | 6.1895 mL | 15.4736 mL | |
Ethanol / DMSO | 10 mM | 0.3095 mL | 1.5474 mL | 3.0947 mL | 7.7368 mL |
15 mM | 0.2063 mL | 1.0316 mL | 2.0632 mL | 5.1579 mL | |
20 mM | 0.1547 mL | 0.7737 mL | 1.5474 mL | 3.8684 mL | |
25 mM | 0.1238 mL | 0.6189 mL | 1.2379 mL | 3.0947 mL | |
30 mM | 0.1032 mL | 0.5158 mL | 1.0316 mL | 2.5789 mL | |
40 mM | 0.0774 mL | 0.3868 mL | 0.7737 mL | 1.9342 mL | |
50 mM | 0.0619 mL | 0.3095 mL | 0.6189 mL | 1.5474 mL | |
60 mM | 0.0516 mL | 0.2579 mL | 0.5158 mL | 1.2895 mL | |
80 mM | 0.0387 mL | 0.1934 mL | 0.3868 mL | 0.9671 mL | |
100 mM | 0.0309 mL | 0.1547 mL | 0.3095 mL | 0.7737 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Chloramphenicol Related Classifications
- Infection Cancer
- Cancer Targeted Therapy Cancer Immunotherapy Cancer Metabolism and Metastasis
- Anti-infection Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK Autophagy Apoptosis MAPK/ERK Pathway PI3K/Akt/mTOR
- Antibiotic Bacterial HIF/HIF Prolyl-Hydroxylase VEGFR Autophagy Apoptosis Beclin1 JNK Akt MMP