Creatinine Urinary Detection Kit
Referência OKAU00002-2PLATE
Tamanho : 2plate
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Application | ELISA-Sandwich | ||||||||||||||||||||||||||||||
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| ELISA Kit Linearity | Linearity was determined by taking two diluted 1:20 human urine samples, one with a low diluted creatinine level of 0.38 mg/dL and one with a higher diluted level of 9.33 mg/dL and mixing them in the ratios given below. The measured concentrations were compared to the expected values.
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| ELISA Kit Principle | The DetectX® Urinary Creatinine Kit is designed to quantitatively measure creatinine present in urine samples. Please read the complete kit insert before performing this assay. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate. The color generating reaction is initiated with the DetectX® Creatinine Reagent, which is pipetted into each well. After a short incubation the intensity of the generated color is detected in a microtiter plate reader capable of measuring 490nm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Four human urine samples were diluted 1:20 with deionized water and run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were:
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| ELISA Kit Component |
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| Additional Information | Background: Creatinine (2-amino-1-methyl-5H-imidazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is < 15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally, altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease. | ||||||||||||||||||||||||||||||
| :: | Detection Limit: 0.037 mg/dL | ||||||||||||||||||||||||||||||
| :: | Cross Reactivity: It is well known that some typical components of human urine may interfere with the Jaffe reaction for creatinine measurement in urine. A diluted urine sample was spiked with 2,000 mg/dL of glucose (equivalent to 40,000 mg/dL undiluted) and tested in the kit. The unspiked diluted sample read at 8.44 mg/dL. No significant change to the measured creatinine level was seen at any glucose concentration. | ||||||||||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||||||||||
| Sample Type | Human, Monkey, Dog, and Rat Urine | ||||||||||||||||||||||||||||||
| Sensitivity | 0.019 mg/dL |
| Gene Full Name | Creatinine Urinary |
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