- More Files
Western Blot (Tissue lysate)
Western blot analysis of CSF1R polyclonal antibody (Cat # PAB3000) in human placenta. CSF1R (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot (Transfected lysate)
(LEFT) Western blot analysis of CSF1R (arrow) using rabbit CSF1R polyclonal antibody (Cat # PAB3000). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CSF1R gene (Lane 2) (Origene Technologies). (RIGHT) Western blot analysis of CSF1R polyclonal antibody (Cat # PAB3000) transiently transfected HEK-293 cell line lysate (1 ug/lane). CSF1R polyclonal antibody (arrow) was detected using the purified polyclonal antibody .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CSF1R polyclonal antibody (Cat # PAB3000) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CSF1R.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CSF1R.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Tissue lysate)
Western blot analysis of CSF1R polyclonal antibody (Cat # PAB3000) in human placenta. CSF1R (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Western Blot (Transfected lysate)
(LEFT) Western blot analysis of CSF1R (arrow) using rabbit CSF1R polyclonal antibody (Cat # PAB3000). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CSF1R gene (Lane 2) (Origene Technologies). (RIGHT) Western blot analysis of CSF1R polyclonal antibody (Cat # PAB3000) transiently transfected HEK-293 cell line lysate (1 ug/lane). CSF1R polyclonal antibody (arrow) was detected using the purified polyclonal antibody .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CSF1R polyclonal antibody (Cat # PAB3000) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. - Gene Info — CSF1R
Entrez GeneID
1436Protein Accession#
P07333Gene Name
CSF1R
Gene Alias
C-FMS, CD115, CSFR, FIM2, FMS
Gene Description
colony stimulating factor 1 receptor
Omim ID
164770Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. [provided by RefSeq
Other Designations
CD115 antigen|FMS proto-oncogene|McDonough feline sarcoma viral (v-fms) oncogene homolog|macrophage colony stimulating factor I receptor|soluble CSF1R variant 1
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Epigenetic consequences of AML1-ETO action at the human c-FMS locus.
Follows GA, Tagoh H, Lefevre P, Hodge D, Morgan GJ, Bonifer C.
The EMBO Journal 2003 Jun; 22(11):2798.
- C-fms expression correlates with monocytic differentiation in PML-RAR alpha+ acute promyelocytic leukemia.
Riccioni R, Saulle E, Militi S, Sposi NM, Gualtiero M, Mauro N, Mancini M, Diverio D, Lo Coco F, Peschle C, Testa U.
Leukemia 2003 Jan; 17(1):98.
- Vitamin D(3) and analogues modulate the expression of CSF-1 and its receptor in human dendritic cells.
Zhu K, Glaser R, Mrowietz U.
Biochemical and Biophysical Research Communications 2002 Oct; 297(5):1211.
- Epigenetic consequences of AML1-ETO action at the human c-FMS locus.