Dipeptidyl Peptidase IV (DPP4) Activity Fluorometric Assay Kit
Referência E-BC-F053-96T
Tamanho : 96T
Marca : Elabscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Detection Principle | Dipeptidyl Peptidase IV (DPP4), also known as CD26, is a kind of serine protease, can decompose the second N-terminal proline or alanine residue of the peptide chain. In organisms, dipeptidyl peptidase can rapidly decompose incretin, which can stabilize insulin level and promote the reduction of blood glucose level in organisms. The detection principle of this kit is that DPP4 can decompose the substrate and release the fluorescent substance AMC, and the activity of this enzyme can be inhibited by adding DPP4 inhibitor. The activity of DPP4 could be calculated by the difference of fluorescence value before and after inhibition. |
| Synonyms | DPP4 |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=360 nm/460 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.92 U/L |
| Detection Range | 0.92-3.77 U/L |
| Precision | inter-assay CV: 5.7% | intra-assay CV: 0.6% |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 5% Mouse kidney tissue homogenate | 4-5 |
| 5% Mouse lung tissue homogenate | 4-5 |
| 5% Mouse heart tissue homogenate | 4-5 |
| 5% Rat liver tissue homogenate | 4-5 |
| 5% Rat brain tissue homogenate | 4-5 |
| Porcine serum | 4-5 |
| Horse serum | 4-5 |
| Dog serum | 4-5 |
| Female chicken serum | 4-5 |
| Macaca fascicularis serum | 4-5 |
| 5.0 × 10^6 HL-60 cell | 1 |
| 4.1 × 10^6 293T cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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