EnzyChrom™ Isocitrate Assay Kit
Application
- For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism.
Key Features
- Fast and sensitive. Linear detection range (20 µL sample): 20 to 5000 µM for 10 min reaction.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD565nm
Samples
- Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species
- All
Procedure
- 10 min
Size
- 100 tests
Detection Limit
- 20 µM
Shelf Life
- 6 months
More Details
- ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost