Feline IL-8 ELISA
Referência DIY1029F-004
Tamanho : 1Set
Marca : Kingfisher Biotech
Feline IL-8 ELISA Data
Feline IL-8 ELISA Kit Components
Component | Usage | Quantity | Catalog # |
Anti-Swine IL-8 Polyclonal Antibody | Capture Antibody | 100 µg X 2 | PB0143S-100 |
Biotinylated Anti-Swine IL-8 Polyclonal Antibody | Detection Antibody | 50 µg | PBB0266S-050 |
Feline IL-8 Recombinant Protein | Standard | 5 µg | RP0108F-005 |
Feline IL-8 ELISA Specifications
The Feline IL-8 Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Feline IL-8 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-8 Background
Interleukin-8 (IL-8), also known as CXCL8, is a CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. There have been 17 different CXC chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2.
Alternate Names - CXCL8, chemokine (C-X-C motif) ligand 8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, IL8, C-X-C motif chemokine ligand 8, Interleukin-8