GBP6 ELISA Kit (Human)

Referência OKEH04629

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH04629
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology’s GBP6 ELISA Kit (Human) (OKEH04629) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GBP6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GBP6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GBP6 captured in the well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-GBP6 Microplate96 Wells (12 x 8 Well Strips)
GBP6 Lyophilized Standard2
Sample Diluent1 x 20 mL
Biotinylated GBP6 Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.88 ng/mL
SpecificityNatural and recombinant Human Guanylate-binding protein 6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGBP6
Gene Full Nameguanylate binding protein family member 6
Alias SymbolsGBP-6;GTP-binding protein 6;guanine nucleotide-binding protein 6;guanylate-binding protein 6.
NCBI Gene Id163351
Protein NameQ6ZN66
Description of TargetGuanylate-binding protein 6
Uniprot IDhttps://www.uniprot.org/uniprot/Q6ZN66
Protein Accession #NP_940862.2
Nucleotide Accession #NM_198460.2
Protein Size (# AA)633
Molecular Weight72 KDa

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90066
 10MG