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> Glutamine synthetase antibody

Glutamine synthetase antibody

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Referência GTX109121-S

Tamanho : 25ul

Marca : GeneTex

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Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121
Anti-Glutamine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Whole mount) (IHC-Wm). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Whole mount) (IHC-Wm). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Immunoprecipitation (IP). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
Anti-Glutamine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109121
Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX109121 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , DDDDK-tagged GLUL-transfected 293T

Predict Reactivity

Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamine synthetase Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950421

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

Cellular Localization

Cytoplasm

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Database

DATA IMAGES

Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121

GTX109121 IHC-P Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample:Paraffin-embedded adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Glutamine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109121

GTX109121 ICC/IF Image

Glutamine synthetase antibody detects Glutamine synthetase protein at astrocytes by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000.

Anti-Glutamine synthetase antibody used in IHC (Whole mount) (IHC-Wm). GTX109121

GTX109121 IHC-Wm Image

Glutamine synthetase antibody detects Glutamine synthetase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Glutamine synthetase antibody (GTX109121) dilution: 1:100.
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Glutamine synthetase antibody detects Glutamine synthetase protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) at a dilution of 1:10000.

Anti-Glutamine synthetase antibody used in IHC (Whole mount) (IHC-Wm). GTX109121

GTX109121 IHC-Wm Image

Glutamine synthetase antibody detects Glutamine synthetase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Glutamine synthetase antibody (GTX109121) dilution: 1:100.
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:20000.

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121

GTX109121 IHC-Fr Image

Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody used in Immunoprecipitation (IP). GTX109121

GTX109121 IP Image

Immunoprecipitation of Glutamine synthetase protein from IMR32 whole cell extracts using 5 μg of Glutamine synthetase antibody (GTX109121).
Western blot analysis was performed using Glutamine synthetase antibody (GTX109121).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121

GTX109121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (GTX109121) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121

GTX109121 IHC-Fr Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 30930743

Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121

GTX109121 IHC-Fr Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 30930743

Anti-Glutamine synthetase antibody used in IHC (Frozen sections) (IHC-Fr). GTX109121

GTX109121 IHC-Fr Image

The data was published in the journal Front Mol Neurosci in 2019. PMID: 30930743

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

The data was published in the journal Front Physiol in 2020.PMID: 32425814

Anti-Glutamine synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109121

GTX109121 ICC/IF Image

The data was published in the journal Sci Rep in 2019. PMID: 31616018

Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121

GTX109121 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35232986

Anti-Glutamine synthetase antibody used in Western Blot (WB). GTX109121

GTX109121 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31616018

Application Reference

Tabor SJ et al. Cells 2023; 12 (13) Retinal Injury Activates Complement Expression in M?ller Cells Leading to Neuroinflammation and Photoreceptor Cell Death.
Xuan DTM et al. Int J Med Sci 2023; 20 (1) : 35-49 Glutamine synthetase regulates the immune microenvironment and cancer development through the inflammatory pathway.
Ludovica Iovino et al. Acta Neuropathol 2022; 144 (1) : 81-106 Trafficking of the glutamate transporter is impaired in LRRK2-related Parkinson's disease.
Application : WB
Reactivity : Human
Gallego-Dur?n R et al. Sci Rep 2022; 12 (1) : 3418 Liver injury in non-alcoholic fatty liver disease is associated with urea cycle enzyme dysregulation.
Application : IHC-P
Reactivity : Human
Carmela Serpe et al. Cells 2021; 10 (8) Microglia-Derived Small Extracellular Vesicles Reduce Glioma Growth by Modifying Tumor Cell Metabolism and Enhancing Glutamate Clearance through miR-124.
Application : IHC
Reactivity : Mouse
Submit a Reference

REVIEW

Glutamine synthetase antibody Cat. No. GTX109121

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Glutamine synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX109121
Date : Anonymous submitted on 25-Feb-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Brain tissue
Blocking : 25°C, 1Hr
Permeabilization /Antigen retrieval : 1%BSA, 15% goat serum/Citrate buffer
Primary Antibody : 1:500, 4°C, 18Hr
Lane Description : Red: Glutamine synthetase, Green: EAAT2, Blue: DAPI
Application Reference
Tabor SJ et al. Cells 2023; 12 (13) Retinal Injury Activates Complement Expression in M?ller Cells Leading to Neuroinflammation and Photoreceptor Cell Death.
Xuan DTM et al. Int J Med Sci 2023; 20 (1):35-49 Glutamine synthetase regulates the immune microenvironment and cancer development through the inflammatory pathway.
Ludovica Iovino et al. Acta Neuropathol 2022; 144 (1):81-106 Trafficking of the glutamate transporter is impaired in LRRK2-related Parkinson's disease.
Application : WB
Reactivity : Human
Gallego-Dur?n R et al. Sci Rep 2022; 12 (1):3418 Liver injury in non-alcoholic fatty liver disease is associated with urea cycle enzyme dysregulation.
Application : IHC-P
Reactivity : Human
Huabo Wang et al. Cell Mol Gastroenterol Hepatol 2021; 12 (1):199-228 Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggressive Murine Hepatoblastomas.
Application : WB
Reactivity : Mouse
Carmela Serpe et al. Cells 2021; 10 (8) Microglia-Derived Small Extracellular Vesicles Reduce Glioma Growth by Modifying Tumor Cell Metabolism and Enhancing Glutamate Clearance through miR-124.
Application : IHC
Reactivity : Mouse
Li Q et al. J Neuroinflammation 2021; 18 (1):271 TRPV4-induced M?ller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway.
Application : IHC-P
Reactivity : Rat
Schmidt A et al. J Cancer Res Clin Oncol 2021; (Epub) Hepatoblastoma: glutamine depletion hinders cell viability in the embryonal subtype but high GLUL expression is associated with better overall survival.
Application : WB
Reactivity : Human
Yamada T et al. Front Physiol 2020; 11:445 Cancer Cachexia Induces Preferential Skeletal Muscle Myosin Loss When Combined With Denervation.
Mundorf A et al. Psychiatry Res 2019; 281:112544 Lithium and glutamine synthetase: Protective effects following stress.
Application : IHC
Reactivity : Mouse
Pronin A et al. Front Mol Neurosci 2019; 12:36 Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.
Nakashima K et al. Sci Rep 2019; 9 (1):14818 A spatial similarity of stereochemical environments formed by amino acid residues defines a common epitope of two non-homologous proteins.
Application : ICC/IF
Reactivity : Human
Kamei Y et al. Am J Physiol Endocrinol Metab 2014; 307 (6):E485-93 FOXO1 activates glutamine synthetase gene in mouse skeletal muscles through a region downstream of 3'-UTR: possible contribution to ammonia detoxification.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

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