Glutathione Peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR):|| GPX GR|2GSH + ROOH -----> GSSG + ROH + H2O, GSSG + NADPH ----> |2GSH + NADP+||Simple, direct and high-throughput assays for GPX activity find wide Applications. The improved assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340nm is directly proportional to the enzyme activity in the sample.||Key Features:|Sensitive and accurate: Use 10ul sample|Linear detection range: 40-800U/L GPX activity||Applications:|Direct Assays: GPX activity in biological samples|Drug Discovery/Pharmacology: Effects of drugs on GPX activity||Kit Components: (100 Tests In 96-Well Plates):|G8127-03A: Assay Buffer, 1x25ml |G8127-03B: GR Enzyme, 1x250ul|*G8127-03C: Glutathione, 1x1vial|*G8127-03D: NADPH, 1x1vial|G8127-03E: H2O2 Solution, 1x50ul |*G8127-03F: Calibrator, 1x1vial||Storage and Stability:|Store all components at -20°C. After reconstitution **G8127-03C, *G8127-03D and *G8127-03F are stable for 3 weeks. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.