Glutathione Reductase (GR) Activity Assay Kit
Referência E-BC-K099-S-100
Tamanho : 100Assays
Marca : Elabscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Detection Principle | With the coenzyme as hydrogen donor, GSSG can be reduced to GSH under the catalysis of GR. Then the GSH content increased and NADPH decreased. The decrease of NADPH absorbance can be measured at 340 nm. The activity of GR can be calculated by detecting the change of NADPH. |
| Synonyms | GR |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (340 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 6.2 U/L |
| Detection Range | 6.2-320 U/L |
| Precision | inter-assay CV: 2.5% | intra-assay CV: 2.1% |
| Sample Volume | 65 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| 10% Mouse liver tissue homogenization | 1-3 |
| 10% Epipremnum aureum tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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