Human Fibronectin
Referência pro-448-200ug
Tamanho : 200ug
Marca : Prospec
Catalogue number
PRO-448
Introduction
Description
Human Fibronectin produced purified from Human Plasma having a Molecular Weight of 440kDa.
Source
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
The Fibronectin was lyophilized from a non sterile 2mg/ml buffer of 10mM sodium phosphate, pH 7.5 and 0.15M NaCl.
Solubility
We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea.
When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).
Stability
Lyophilized Fibronectin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibronectin should be stored at 4°C between 2-7 days and for future use below -18°C.
Purity
≥ 95.0% as determined by SDS-PAGE.