Human STEAP1 antibody
Referência AST0910-50
Tamanho : 50ul
Marca : NKMAX BIO
Contactar o distribuidor local :
Telefone : +1 850 650 7790
ICC/IF analysis of STEAP1 in LNCap cells. The cell was stained with AST0910 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(30ug) and tissue lysates(30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: LNcap cell lysate
Lane 2.: Mouse Testis Tissue lysate
Lane 1.: LNcap cell lysate
Lane 2.: Mouse Testis Tissue lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.