Hydroxyproline (Hyp) Colorimetric Assay Kit (Alkali hydrolysis Method)

Referência E-BC-K061-S-50

Tamanho : 50Assays

Marca : Elabscience

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Detection Principle The oxidation product which produced by hydroxyproline under the action of oxidant react with dimethylaminobenzaldehyde and show a purplish red color. The content of hydroxyproline can be calculated by measuring the OD value at 550 nm.
Synonyms Hyp
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (565 nm)
Research Area Amino Acids And Proteins
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.024 μg/mL
Detection Range 0.024-5 μg/mL
Precision inter-assay CV: 0.7-2.0% | intra-assay CV: 3.3-4.2%
Assay Time 2 h
Cat.No. Product Name Sizes
E-BC-K062-S Hdroxyproline (HYP) Colorimetric Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Rat serum 1
Rat plasma 1
Fish scale 4-10
Fin tissue 4-10
Fishtail tissue 4-10
Mouse lung tissue 1
Mouse muscle tissue 2-5
Mouse leg bone tissue 4-10

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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