K1205-1 | HyperFluor™ 488 Goat Anti-Human IgG (H+L) Antibody Quimigen

General Information

Name	HyperFluor™ 488 Goat Anti-Human IgG (H+L) Antibody
Host species	Goat
Target species	Human
Tested applications	Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA
Conjugation	Alexa 488. Ex: 495 nm, Em: 519 nm

Product Citation

1. Jing Lu, et al. "Effectiveness of a broad-spectrum bivalent mRNA vaccine against SARS-CoV-2 variants in preclinical studies." Emerg Microbes Infect. 2024 Dec;13(1):2321994. PMID: 38377136

Description

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for Human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Quality Control

Datasheet

Applications

Application	Notes
ICC/IF	1/500-1/2000
IHC-Fr	Use at an assay dependent dilution.
IHC-P	1/100-1/500
Flow Cyt	1/250-1/1000
ELISA	Use at an assay dependent dilution.

Properties

Form	Liquid
Storage buffer	Preservative: 0.02% Sodium azide Constituents: 23% Glycerol, PBS, 1% BSA
Storage instructions	Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Keep away from Light.
Concentration	1 mg/mL
Purity	Immunogen affinity purified
Purification notes	This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Clonality	Polyclonal
Isotype	IgG

Storage

Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Keep away from Light.

HyperFluor™ 488 Goat Anti-Human IgG (H+L) Antibody

Referência K1205-1

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Marca : APExBIO Technology