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Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

KDM5B monoclonal antibody (M02), clone 1G10

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Referência H00010765-M02

Tamanho : 100ug

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Abnova

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Specifications

Product Description

Mouse monoclonal antibody raised against a partial recombinant KDM5B.MAB,monoclonal Ab,monoclonal antibody

Immunogen

KDM5B (NP_006609, 231 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence

AKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGN

Host

Mouse

Reactivity

Human

Interspecies Antigen Sequence

Mouse (94); Rat (93)

Isotype

IgG2a Kappa

Quality Control Testing

Antibody Reactive Against Recombinant Protein.

Western Blot detection against Immunogen (35.53 KDa) .

Storage Buffer

In 1x PBS, pH 7.4

Storage Instruction

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Applications

Western Blot (Recombinant protein)

Protocol Download

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KDM5B on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
Protocol Download

Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KDM5B is approximately 0.03ng/ml as a capture antibody.
Protocol Download

ELISA

Immunofluorescence
Immunofluorescence of monoclonal antibody to KDM5B on HeLa cell . [antibody concentration 10 ug/ml]
Protocol Download
Gene Info B

Entrez GeneID
10765

GeneBank Accession#
NM_006618

Protein Accession#
NP_006609

Gene Name

KDM5B

Gene Alias

FLJ10538, FLJ12459, FLJ12491, FLJ16281, FLJ23670, KDM5B, PLU-1, PUT1, RBBP2H1A

Gene Description

jumonji, AT rich interactive domain 1B

Omim ID
605393

Gene Ontology
Hyperlink

Gene Summary

O

Other Designations

OTTHUMP00000038815|RBP2-like|putative DNA/chromatin binding motif|retinoblastoma-binding protein 2, homolog 1A
Interactomes
- KDM5B
Diseases
- Genetic Predisposition to Disease
- Hypertension
Publication Reference
- The KDM5B and KDM1A lysine demethylases cooperate in regulating androgen receptor expression and signalling in prostate cancer.
  Veronika M Metzler, Simone de Brot, Daisy B Haigh, Corinne L Woodcock, Jennifer Lothion-Roy, Anna E Harris, Emeli M Nilsson, Atara Ntekim, Jenny L Persson, Brian D Robinson, Francesca Khani, Kristian B Laursen, Lorraine J Gudas, Michael S Toss, Srinivasan Madhusudan, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan, Jennie N Jeyapalan.
  
  Frontiers in Cell and Developmental Biology 2023 Apr; 11:1116424.
  
  Application：IHC, Human, prostate.
- Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma.
  Shigekawa Y, Hayami S, Ueno M, Miyamoto A, Suzaki N, Kawai M, Hirono S, Okada KI, Hamamoto R, Yamaue H.
  
  Oncotarget 2018 Sep; 9(76):34320.
  
  Application：IHC, Human, Human hepatocellular carcinoma, HLF, HepG2, Huh7 cells.
- Jumonji/ARID1 B (JARID1B) Protein Promotes Breast Tumor Cell Cycle Progression through Epigenetic Repression of MicroRNA let-7e.
  Mitra D, Das PM, Huynh FC, Jones FE.
  
  The Journal of Biological Chemistry 2011 Nov; 286(4):40531.
  
  Application：IP-WB, Human, MCF-7, T47D cells.
- Overexpression of the JmjC histone demethylase KDM5B in human carcinogenesis: involvement in the proliferation of cancer cells through the E2F/RB pathway.
  Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T, Field HI, Kelly JD, Neal DE, Yamaue H, Ponder BA, Nakamura Y, Hamamoto R.
  
  Molecular Cancer 2010 Mar; 9:59.
  
  Application：IHC, WB, IF, Human, Bladder, lung tumor, A549 and SBC5 cells.
- JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.
  Xiang Y, Zhu Z, Han G, Ye X, Xu B, Peng Z, Ma Y, Yu Y, Lin H, Chen AP, Chen CD.
  
  PNAS 2007 Nov; 104(49):19226.

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