Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Referência E-BC-K025-M-96T

Tamanho : 96T

Marca : Elabscience

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Detection Principle MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Synonyms MDA
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (530-540 nm, optimum wavelength: 532 nm)
Research Area Oxidative Stress ,  Ferroptosis
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 1.13 μmol/L
Detection Range 2.92-40 μmol/L
Precision inter-assay CV: 7.2% | intra-assay CV: 4.1%
Assay Time 1 h 10 min
Cat.No. Product Name Sizes
E-BC-K020-M Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) 500Assays , 96T , 48T
E-BC-K031-M Catalase (CAT) Activity Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Human plasma 1
Rat serum 1
Rat plasma 1
Mouse serum 1
Mouse plasma 1
10% Rat heart tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat brain tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Referência
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Price Bef. VAT
4800-SC
 5x10^5cells/vial