Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

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Referência E-BC-K025-S-100

Tamanho : 100Assays

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Elabscience

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Detection Principle	MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Synonyms	MDA
Sample Type	Serum,Plasma,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (532 nm)
Research Area	Oxidative Stress
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.38 nmol/mL
Detection Range	0.38-133.33 nmol/mL
Precision	inter-assay CV: 0.08 \| intra-assay CV: 4.9%
Assay Time	1 h 30 min

Cat.No.	Product Name	Sizes
E-BC-K031-S	Catalase (CAT) Activity Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Rat serum	1
Rat plasma	1
Mouse serum	1
Mouse plasma	1
10% Rat heart tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.