Mouse anti Human IgG2 (Fc subclass specific)

Background
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc portion of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Source
Highly purified monoclonal IgG2 isolated from pooled human serum

Product
Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.

Formulation: Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added.

Specificity
Monoclonal Mouse antibody to Human IgG2, subclass specific.

Species Reactivity: The antiserum does not react with any other component of the Human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Applications
Indirect immunofluorescence,ELISA.

Storage
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
Evaluation of monoclonal antibodies having specificity for human IgG subclasses: Results of the 2nd IUIS/WHO collaborative study. Immunology Letters 31 (1992), 143-168. Jefferis R, Reimer C B, Skavril F, de Lange G, Goodall D M, Bentley T L, Phillips D J, Vlug A, Harada S, Radl J , Claassen E, Boersma J A, and Coolen J.