Mouse Anti-Mouse IgM Monoclonal Antibody (clone: 7C2C6C2) PE conjugated
Referência CFF8263-100
Tamanho : 100Assays
Marca : Cohesion Biosciences
Product Name: | Mouse IgM Isotype Control-PE labled |
Cat No: | CFF8263 |
Source: | Mouse |
Reactivity: | |
Applications: | IF, FC |
*Application Key: | |
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
*Species Reactivity Key: | |
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference |
Description:Mouse IgM Isotype Control-PE labled can be used as an isotype control in flow cytometry and other applications.Purification:The antibody was purified by affinity chromatography.Clonality:Monoclonal (clone: 7C2C6C2)Conjugation:PEForm:Mouse IgM. Liquid in PBS, pH 7.3, and 0.02% sodium azide.Dilution:10 μl / assay Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.
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