Mouse Anti-Plakoglobin Monoclonal Antibody (Clone:PG 5.1)

Referência 690005

Tamanho : 1ml(50µg/ml)

Marca : Progen

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anti-Plakoglobin mouse monoclonal, PG 5.1, liquid, purified

Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Dog, Human, Mouse, Rat, Zebrafish
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone PG 5.1
Immunogen Plakoglobin, "band 5" protein from bovine snout epidermis
Concentration 50 µg/ml (50 µg)
Formulation PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5 (10 µg/ml)
Immunohistochemistry (IHC) - frozen 1:25-1:100 (0.5-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:100 (0.5-2 µg/ml, microwave treatment recommended)
Western Blot (WB) 1:500-1:2,000 (0.025-0.1 µg/ml)

PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide reacting: Mr 83,000 Plakoglobin, 'band 5' polypeptide of intercellular adhering junctions (identical to gamma-catenin).

Reactivity on cultured cell lines: cell cultures forming monolayers.

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