600184 | Mouse G-CSF Quimigen Portugal

Recombinant Active Protein

Cat. No.

600184

Unit

0.01 mg

Description

Granulocyte colony-stimulating factor (G-CSF) induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. Secretion of G-CSF in epithelial, endothelial, and fibroblastic cells is induced by Interleukin-17. Recombinant Mouse G-CSF produced in E. coli is a single non-glycosylated polypeptide chain containing 179 amino acids with a MW of 19 kDa.

Spec Container

Datasheet

Format

Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

Granulocyte colony-stimulating factor; G-CSF; CSF3; GCSF; Csfg

Accession No.

P09920

19000 Da

Sequence

MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Mouse recombinant G-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of mouse M-NFS-60 cells, is 0.04 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Mouse G-CSF

Referência 600184

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Marca : Abbiotec

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Spec Container