Mouse Ig, Human ads Antibody : Biotin
Referência OASB01454
Tamanho : 0.5mg
Marca : Aviva Systems Biology
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Goat Anti-Mouse Ig Antibody - Biotin Conjugated (OASB01454)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse Ig Antibody - Biotin Conjugated (OASB01454) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, ELISPOT, ICC, WB |
| Additional Information | Description: Pepsin digest of Goat Anti-Mouse Ig, Human ads - Pooled antisera from goats hyperimmunized with mouse IgG, IgM, and IgA; purified by affinity chromatography on mouse IgG, IgM, and IgA covalently linked to agarose; reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA; cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgG, IgM, and IgA |
| Purification | Gel filtration chromatography of pepsin digested antibody. |
| Concentration | 0.5 mg/mL |
| Specificity | Ig |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance with characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: FITC conjugate BIOT conjugate R-PE, APC, SPRD, CY5 and R-PE-CY5.5 conjugates AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Indirect immunofluorescent staining in conjunction with mouse monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3 Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), and R- phycoerythrin- Cyanine5.5 (R- PE- CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Pooled human sera and purified human paraproteins. |
| Gene Full Name | B-cell antigen receptor complex-associated protein alpha chain |
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