Mouse IgD Antibody : PE
Referência OASB00163
Tamanho : 0.1mg
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
Mouse IgD Antibody : PE (OASB00163)
| Datasheets/Manuals | Printable datasheet for Mouse IgD Antibody : PE (OASB00163) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 11-26 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | R-PE |
| Application | FC |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | 0.1 mg/mL |
| Specificity | IgD |
| Characterization | To insure acceptable performance, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent. Representative data are included with this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1-5 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: Ug/106 cells BALB/c splenocytes were double-stained with rat anti-mouse IgD-FITC and rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
