Mouse IgE Antibody : Biotin
Referência OASB01647
Tamanho : 1.0mg
Marca : Aviva Systems Biology
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Goat Anti-Mouse IgE Antibody - Biotin Conjugated (OASB01647)
| Datasheets/Manuals | Printable datasheet for anti-IGHE (OASB01647) antibody |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, ICC, WB, IP |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with mouse IgE; purified by affinity chromatography on mouse IgE covalently linked to agarose; reacts with the heavy chain of mouse IgE; cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgE |
| Purification | Affinity chromatography on mouse IgE covalently linked to agarose. |
| Concentration | 0.5 mg/mL |
| Specificity | IgE |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA for conformance to characteristicsof a standard reference reagent. This product is not tested for reactivity to other species IgE. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | ELISA: AP conjugate HRP conjugate BIOT conjugate 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Indirect immunostaining in conjunction with mouse IgE monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - Reagents are stable for the period shown on the label if stored as directed. |
| Cross Absorption | Mouse IgG1, IgG2a, IgG2b, IgG3, IgM and IgA. |
