Mouse Monoclonal Anti-Keratin K13, lyophilized, purified [Clone Ks13.1]
Referência 61007
Tamanho : 50µg
Marca : Progen
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| Quantity | 50 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Bovine, Human, Rat |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | Ks13.1 |
| Immunogen | Keratin K13 of Mr 54,000 purified from human esophagus |
| Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Synonym | Cytokeratin 13 |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:5-1:25 (2 µg/ml - 10 µg/ml) |
| Immunohistochemistry (IHC) - paraffin | 1:5-1:25 (2 µg/ml - 10 µg/ml) (protease treatment and/or microwave treatment recommended) |
| Western Blot (WB) | 1:50-1:100 |
Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin.
Polypeptide reacting: Mr 54,000 polypeptide human keratin K13 (formerly designated cytokeratin 13; with minor affinity to keratins K14, Mr 50,000, and K16, Mr 48,000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle).
Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma.
Reactivity on cultured cell lines: cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder CA.
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