MTT Cell Proliferation Assay Kit is a widely used cell proliferation and toxicity assay kit. MTT, a tetrazolium salt, can be reduced by succinate dehydrogenase in the mitochondria of living cells to the water-insoluble purple formazan crystals. The amount of formazan can be quantified by measuring the absorbance at 570 nm upon solubilization and is proportional to the number of living cells.
This kit provides a special formazan solvent, which does not need to remove the original medium and can be directly added to dissolve the formazan. At the same time, this kit is easy to use, highly sensitive and has a good linear range.
Figure 1: Figure 1: Reference standard curve of this kit